Journal: mBio

Article Title: Mucosal Bacteria Modulate Candida albicans Virulence in Oropharyngeal Candidiasis

doi: 10.1128/mBio.01937-21

Figure Lengend Snippet: Relationship between Lactobacillus and Enterococcus as revealed by microbiome SparCC network analysis and coculture experiments. (A and B) SparCC networks highlighting the 5 most abundant OTUs (OTU0001, Enterococcus faecalis ; OTU0002, Streptococcus mitis ; OTU0003, Lactobacillus johnsonii ; OTU0004, Staphylococcus saprophyticus ; and OTU0012, Streptococcus sanguinis ) in immunosuppressed Candida -infected mice. The five most abundant OTUs identified by species level are color coded, while “others” are white. Node size is scaled based on the overall abundance of each OTU in the microbiota. Edge width is proportional to the strength of association between each OTU pair (as measured by the correlation coefficient), red edge indicates a negative correlation, and green edge indicates a positive correlation. (A) SparCC analysis in Candida -infected mice; (B) SparCC analysis in Candida -infected mice receiving sucrose. Lactobacillus johnsonii maintained a significant negative correlation with Enterococcus faecalis regardless of sucrose treatment ( P < 0.001, r = −0.64, and P < 0.001, r = −0.58, with and without sucrose, respectively). (C) Two murine isolates ( L. johnsonii and E. faecalis ) were grown in coculture in MRS broth (without dextrose) supplemented with 2% glucose or 2% sucrose anaerobically for 24 h. Aliquots were plated on Rogosa (for lactobacilli) or CATC (for enterococci) agar for quantitative culture. Bold font on the x axis indicates the organism whose mean CFU is shown, error bars are SDs of the means from triplicate samples in two independent experiments. CFU counts of E. faecalis were significantly lower after 24 h of coculture with L. johnsonii , regardless of the carbon source. ****, P = 0.001.

Article Snippet: All Enterococcus isolates were identified as Enterococcus faecalis (99.19% similarity and maximum bit-score of 2,139 for strains NBRC 1000481 and ATCC 19433).

Techniques: Infection

Journal: Microbiology Spectrum

Article Title: GVPC Medium Manufactured without Oxygen Improves the Growth of Legionella spp. and Exhibits Enhanced Selectivity Properties

doi: 10.1128/spectrum.02401-21

Figure Lengend Snippet: Comparison of growth in GVPC manufactured with O 2 removal and N 2 injection during the ingredient mixture at 115°C, 15 min. (A) Enterococcus faecalis ATCC 19433 growth after incubation at 36 ± 2°C for 3 days in GVPC manufactured in different conditions: (a) 115°C, 15 min, without O 2 (conditions 3); (b) normal conditions (conditions 1); (c) 115°C, 15 min (conditions 2). (B) Enterococcus faecalis ATCC 29212 growth after incubation at 36 ± 2°C for 3 days in GVPC manufactured in different conditions: (a) 115°C, 15 min, without O 2 (conditions 3); (b) normal conditions (conditions 1); (c) 115°C, 15 min (conditions 2). (C) Escherichia coli ATCC 25922 growth after incubation at 36 ± 2°C for 3 days in GVPC manufactured in different conditions: (a) 115°C, 15 min, without O 2 (conditions 3); (b) normal conditions (conditions 1); (c) 115°C, 15 min (conditions 2) (D) Pseudomonas aeruginosa ATCC 9027 growth after incubation at 36 ± 2°C for 3 days in GVPC manufactured in different conditions: (a) 115°C, 15 min, without O 2 (condition 3); (b) normal conditions (conditions 1); (c) 115°C, 15 min (conditions 2). (E) Pseudomonas aeruginosa ATCC 9027 growth after incubation at 36 ± 2°C for 3 days in GVPC manufactured in different conditions: (a) 115°C, 15 min, without O 2 , N 2 injection (conditions 4); (b) normal conditions (conditions 1); (c) 115°, 15 min, without O 2 (conditions 3).

Article Snippet: In the selectivity assays, complete inhibition of growth was observed for E. faecalis (both strains ATCC 29212 and ATCC 19433), E. coli (ATCC 25922), and P. aeruginosa (ATCC 9027).

Techniques: Comparison, Injection, Incubation